|
KMID : 0378019900330120058
|
|
New Medical Journal
1990 Volume.33 No. 12 p.58 ~ p.66
|
|
|
Subtype Analysis of T Lymphocytes from Neonates
|
|
|
|
|
Abstract
|
|
|
This study was designed to evaluate the immune cellular defects indirectly from the blood of neonates who have an increased risk of severe infections by using monoclonal antibodies(Mab) and flow cytometer. The author compared cell surface on cord blood lymphocytes(CBL) from 10 term infants with standard as well as newly defined Mab that distinguish regulatory T cells. Cord blood contained more white blood cells(WBC) than peripheral bloods(PBL) from other age groups, but the absolute numbers of lymphocytes of cord blood were the same with those of infants and adults. CBL had small percentages than lymphocytes of childhood(7?8 age) that express the CD3 marker(total T cells), although absolute numbers of CD3+ cell were comparable in neonates and other age groups. CBL and PBL were similar in terms of the percentage of CD4+ cells(helper /inducer T cells), although the absolute numbers of CD4+ cells were higher in CBL than in PBL. CBL had significantly smaller percentages and lower absolute numbers of CD8+ cells (suppressor. cytotoxic T cells) than PBL, and the ratio of CD4+ and CD8+ cells was highest in the CBL among PBL of various age groups. The CD4+ population was subdivided into cells bearing the suppressor-inducer and helper-inducer populations, which have recently been reinterpreted as naive and memory maturation states, using anti-2H4 Mab and dual parameter analysis with anti-CD4.
The absolute numbers and percentages of CD4+ and 2H4+ naive T cells were higher in CBL than in PBL. This data suggest that the predominance of 2H4+ naive T cells in the CD4 population whose function is associated with that of the induction of suppression rather than the up-regulation of immune responses may increased susceptibility of neonates to infection.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|